Characterization of [125I]-endothelin-1 and [125I]-BQ3020 binding to rat cerebellar endothelin receptors

Receptor Autoratiographic Evidence for Specific 125I-Endothelin-1 Binding Sites in the Rat Eye

Specific 125I-endothelin-1 (125I-ET-1) binding sites were investigated in the rat eye, using receptor autoradiographic techniques. 125I-ET-1 was specifically bound to the rat eye sections, with a slow association rate, as evidenced by kinetic experiments. The radioligand binding reached a maximum at 48hr of incubation and a plateau was maintained for up to 72hr. No degradation of 125I-ET-1 duri...

Rat Cerebellar Grafts Have 125I-Vasoactive Intestinal Peptide Binding Sites

Neuropeptides and their receptors may play a role in the growth and/or differentiation of fetal brain transplants. Previously, we found that gas-trin releasing peptide (GRP) receptors develop on fetal cortex grafts transplanted into the adult rat cortex, spinal cord or 4th ventricle/1,2/. Here we investigated the presence of vasoactive intestinal peptide (VIP) receptors in rat fetal brain trans...

Characterization of 125I-tissue plasminogen activator binding to cerebellar granule neurons

Mouse cerebellar cells in culture secrete tissue plasminogen activator (tPA) into the culture medium. Fibrin overlays have shown tPA to be associated with granule neurons in these cultures. This cell associated tPA can be displaced by extensive washing of the cells or by a brief lowering of the pH (less than 4), which leads to a loss of fibrinolytic activity by the cells. Incubation of these fi...

Binding of 125I-labelled vasopressin to isolated rat hepatocytes.

Characterization of [125I]sauvagine binding to CRH2 receptors: membrane homogenate and autoradiographic studies.

We describe the binding of [125I]tyr(o)sauvagine to membranes of corticotropin-releasing hormone (CRH2) receptor expressing HEK293/EBNA (293ECRH2 alpha) cells. The binding of [125I]tyr(o)sauvagine to CRH2 receptors was temperature, time and tissue dependent. Equilibrium was reached in 2 hr at 23 degrees C. Saturation data best fit a two-site model with affinity constants of 44 pM and 4.1 nM for...